ACLY Monoclonal Antibody-MB66502

ACLY Monoclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: MB66502-50, MB66502-100 Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Swiss-Prot: P53396 Host: Mouse Reactivity: Human, Mouse, Monkey Applications: WB, IF/ICC, FC All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), FC (1/50 - 1/100) Background: ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis . Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase . It is phosphorylated by GSK-3 on Thr446 and Ser450 . Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt . Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme . Purification and