hGH ADA (human growth hormone) ELISA - EL-141-878

hGH ADA (human growth hormone) ELISA Catalogue Number: EL-141-878 Size: 1X96 wells Antigen: antibodies to hGH Storage: -20°C, 1 year Reactivity: Human Detection Range: 30 ng/mL -1920 ng/mL Detection Minimum: 30 ng/mL Protocol: This assay employs the indirect enzyme immunoassay technique. hGH is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to hGH present in biological matrices are bound to the immobilized hGH. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to hGH present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured. Components: Coated microtiter plate, 96 wells (12x8 strips) 1 Calibrator diluent 1.8 ml Calibrator (1.15mg/mL) 12 μl 10X wash buffe