Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit - E-CL-R0485

Rat NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) CLIA Kit Sizes: 24T, 48T, 96T Catalogue Numbers: E-CL-R0485-24, E-CL-R0485-48, E-CL-R0485-96 Citations, Manuals and MSDS Available upon request. Abbreviation: NT-proBNP UNIProt ID: P13205 Target Synonyms: NT-Pro-BNP Research Areas: Cancer, Cardiovascular, Neuroscience Target Species: Rat Application: CLIA Usage: This ELISA kit applies to the in vitro quantitative determination of Rat NT-proBNP concentrations in serum, plasma and other biological fluids. Tested Sample Types: Serum, Plasma and other biological fluids Type: Sandwich Assay Time: 3.5h Detection Type: Chemiluminescence Sensitivity: 7.5 pg/mL Detection Range: 12.5-800 pg/mL Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-proBNP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat NT-proBNP. You can calculate the concentration of Rat NT-proBNP in the samples by comparing the RLU value of the samples to the standard curve. Cross Reactivity: This kit recognizes Rat NT-proBNP in samples. No significant cross-reactivity or interference between Rat NT-proBNP and analogues was observed Recovery Rate: 80%-120% Precision: Both intra-CV and inter-CV are < 10%. Storage: 2-8℃/-20℃, 6 months Shipping: ice bag