Recombinant Human Sonic HedgeHog (Shh) - PR1114

Recombinant Human Sonic HedgeHog (Shh) Catalogue Numbers: PR1114-5, PR1114-25 Sizes: 5µg, 25µg Source: Escherichia coli Molecular Weight: Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids. Purity: >98% by SDS-PAGE and HPLC analyses. Biological Activity: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml, corresponding to a Specific Activity of ＞1 x 103 IU/mg. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder. Formulation: Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl. AA Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG Endotoxin: Less than 1EU/µg of rHuSHH as determined by LAL method. Reconstitution: We recomme