RedCushion®, For Protection and Higher Recovery in Semen Centrifugation, 100ml, Each

RedCushion is a high-density colloidal medium used in semen centrifugation to protect sperm cells, reducing damage caused by conventional centrifugation. RedCushion- centrifugation semen produces less compacted pellets and a high sperm recovery rate. Due to its reddish color, it allows easy visualization of the pellet, as well as its removal. Directions for usage (1) 1 – Aspire 3 mL of RedCushion with a new syringe and needles, and deposit it at the bottom of the centrifugation tube; 2 – Dilute the ejaculate with BotuSemen in the proportion of 1:1 v/v; 3 – Incline the tube to 45º and slowly deposit the diluted semen on the wall with plastic Pasteur pipettes so that no mixing occurs between the semen and the RedCushion; 4 – Centrifuge at 1000 xg for 20 minutes (Fanem centrifuges: 1000 xg = 2,700 rpm); 5 – With the help of a pipette, remove the sperm “ring” deposited on top of RedCushion; 6 – Re-suspend the sperm with BotuSemen or BotuCrio, according to the procedure to be held. Directions for usage (2) 1 – Dilute the ejaculate with BotuSemen in the proportion of 1:1 v/v; 2 – Insert the diluted semen into a centrifuge tube; 3 – With the help of a new syringe and needles, deposit 3 mL of RedCushion under the diluted semen; 4 – Centrifuge at 1000 xg for 20 minutes (Fanem centrifuges: 1000 xg = 2,700 rpm); 5 – Remove the supernatant; 6 – Aspire the formed pellet over the RedCushion; 7 – Re-suspend the sperm with BotuSemen or BotuCrio, according to the procedure to be held.