AmScope IN300 Series Inverted Epi-fluorescence Trinocular Compound Microscope

The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity. The Viewing Head The viewing head has a 45&deg incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces. OpticsThe infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness. The Stage The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. Transmitted Illumination The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction. Phase Contrast The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light. Epi-fluorescence Illumination For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available. Fluorescence Filters Filter Set Excitation Filter Dichromatic Mirror--> Emission Filter Common Stains--> B 450-490nm --> 520nm Acridine Orange (DNA), Acridine Yellow, Alexa Fluor (430, 488, 500, 514),Cy2, DiO, FITC, Lucifer Yellow--> G 510-560nm --> 590nm Acridine Red, Alexa Fluor 532, NeuroTrace 530/615, Rhodamine B--> Mercury HBO Excitation Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths. Band Peak Wavelength UV 334nm, 365nm V 405nm B 436nm G 546nm Y 578nm Digital ImagingCapture photos and videos with the included 5MP color-CCD camera. The camera is designed for low-light applications like fluorescence, favoring sensitivity over high resolution. The active cooling system helps regulate dark noise to improve image quality. The included Windows software allows you to monitor live images from your microscope, and record still images and video. An assortment of tools can be used to optimize the image before and after capture. Specifications Optical System Infinity-corrected Reference Focal Length --> Head Trinocular, 45° incline Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm Ocular Diameter 30mm Ocular Diopters right ocular Eyepieces 10X Photo-port height-adjustable 23mm tube Objective Lenses Infinity-corrected Objective Turret Quadruple Focusing System Focus Adjustment Coaxial Coarse/Fine, adjustable tension Fine Division 0.002mm Coarse Stroke--> Fine Stroke0.2mm--> Stage fixed-height, mechanical X-Y translation Stage Dimensions 172mm x 242mm Stage X-Y Travel Range 75mm x 40mm Specimen Holders Terasaki plate, Petri dish Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable Transmitted Illumination 12V 30W halogenvariable intensity Condenser NA 0.3, iris diaphragm, filter slot Condenser Working Distance --> Phase Contrast Annuli --> Power 110VAC 60Hz Objective Lenses Magnification Corrections NA Working Distance Cover-glass Thickness 4X plan fluor 0.15 28mm 1.5mm 10X plan fluor, phase-contrast 0.30 3mm 1.5mm 20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm 40X plan fluor 0.60 2.23mm 1.5mm Camera Specifications Sensor Type Interline CCD (color) Sensor Optical Format 2/3" Active Pixels 5M (2580 x 1944) Pixel Size 3.4µm x 3.4µm Active Sensor Area 9.74mm x 7.96mm Shutter global shutter Sensitivity 280mV @ 1/30s Spectral Response --> Capture Resolution and Maximum Framerate 3fps @ 2580x194410fps @ 1280x932 Connectivity USB 2.0 Lens-mount C-mount Compensating Lens 0.67X/0.75X Cooling Type Peltier Thermoelectric Cooling -30°C below ambient Power --> Software OS Requirements Windows (32/64 bit) 7/8/10 Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM --> Features Windows Version Live video preview Captures still images and video Exposure and white-balance controls Image processing Measurement tools, including lines, arcs, polygons Packing List:One microscope body Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X One pair 10X eyepieces One phase-contrast annulus board One top-mount illuminator One rear-mount fluorescence illuminator One power supply for fluorescence illuminator One UV blocking shield One 30W halogen bulb One 100W mercury bulb One dust cover Color filters Two power cordsOne C-mount TEC USB camera One AC power adapter (North America plug) One USB 2.0 cable One software CD One case