BspQI GMP-grade (10 U/μL)

Description This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly (A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends. This product is produced in accordance with GMP process requirements and provided in a liquid form. Feature Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence Restriction Enzyme Cut Site: GCTCTTC (1/4) Digestion of DNA to obtain specific sticky ends Isolated from a recombinant source Tested for the absence of endonucleases, exonucleases, RNases Application Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G); Linearize plasmid template before in vitro transcription Restriction digest Specification Expression Host Recombinant E. coli with BspQI gene Re