Random Hexamers primer

Random Hexamers are short oligodeoxyribonucleotides of random sequences [d(N)6] that anneal to random complementary sites on a target DNA or RNA and serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase.During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between the mRNA and other RNA species present in the reaction. ProtocolWe recommend using 1 μl of Random Hexamers primer per 20 μl reaction volume. Quality controlThis product is qualified in a cDNA reaction.