Recombinant Bovine Glycolipid Transfer Protein (GLTP) Protein (His)

Product Overview Description Recombinant Bovine Glycolipid Transfer Protein (GLTP) Protein (His) is produced by our Yeast expression system. This is a full length protein. Purity Greater than 85% as determined by SDS-PAGE. Uniprotkb P68265 Target Symbol GLTP Synonyms GLTPGlycolipid transfer protein; GLTP Species Bos taurus (Bovine) Expression System Yeast Tag N-6His Target Protein Sequence ALLAEHLLRPLPADKQIETGPFLEAVSHLPPFFDCLGSPVFTPIKADISGNITKIKAVYDTNPTKFRTLQNILEVEKEMYGAEWPKVGATLALMWLKRGLRFIQVFLQSICDGERDENHPNLIRVNATKAYEMALKKYHGWIVQKIFQAALYAAPYKSDFLKALSKGQNVTEEECLEKVRLFLVNYTATIDVIYEMYTRMNAELNYKV Expression Range 2-209aa Protein Length Full Length of Mature Protein Mol. Weight 25.8kD Research Area Signal Transduction Form Liquid or Lyophilized powder Buffer Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Target Details Target Function Accelerates the intermembrane transfer of various glycolipids. Catalyzes the transfer of various glycosphingolipids between membranes but does not catalyze the transfer of phospholipids. May be involved in the intracellular translocation of glucosylceramides. Subcellular Location Cytoplasm. Protein Families GLTP family Database References KEGG: bta:327678 STRING: 9913.ENSBTAP00000003355 UniGene: Bt.48472 Tissue Specificity Detected in brain, kidney, spleen, lung, cerebellum, liver and heart. Gene Functions References three different X-ray structures of bovine GLTP at 1.4 angstroms, 1.6 angstroms and 1.8 angstroms resolution, all with a bound fatty acid or glycolipid, were solved. PMID: 16309699