Recombinant Cynomolgus/Rhesus macaque CD47 Protein, Active

Product Overview Description Recombinant Cynomolgus/Rhesus macaque CD47 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Glu141. Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC Accession F7A802 Target Symbol CD47 Species Cynomolgus/Rhesus macaque Expression System HEK293 Tag C-His Expression Range Gln19-Glu141 Mol. Weight The protein has a predicted MW of 15 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result. Form Lyophilized Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Endotoxin Less than 1EU per ug by the LAL method. Activity Immobilized Cynomolgus/Rhesus macaque CD47, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). The affinity constant of 63.27 nM as dete