Recombinant Human CA125/MUC16 Protein, Active

Product Overview Description Recombinant Human CA125/MUC16 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly12660-Met12923. Purity > 95% as determined by Tris-Bis PAGE Accession Q8WXI7 Target Symbol CA125/MUC16 Synonyms CA125; CA-125; CA125MUC-16; FLJ14303; MUC16 Species Human Expression System HEK293 Tag C-His-Avi Expression Range Gly12660-Met12923 Mol. Weight The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 50-80 kDa based on Tris-Bis PAGE result. Form Lyophilized Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Endotoxin Less than 1EU per ug by the LAL method. Activity Immobilized Human CA125, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 1.13ug/ml determined by ELISA. Contact us for detailed testing images. Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Shipping Shipped at ambient temperature. Gene Background MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.