Recombinant Human CD47 Protein, Active

Product Overview Description Recombinant Human CD47 is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Pro139. Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC Accession Q08722 Target Symbol CD47 Synonyms CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 Species Human Expression System HEK293 Tag C-His Expression Range Gln19-Pro139 Mol. Weight The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. Form Lyophilized Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Endotoxin Less than 1EU per ug by the LAL method. Activity Immobilized Human CD47, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 82.1ng/ml determined by ELISA (QC Test). The affinity constant of 18.9nM as deter