Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein, Active

Product Overview Description Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152. Purity > 95% as determined by Tris-Bis PAGE Accession P10747 Target Symbol CD28 Synonyms CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290 Species Human/Cynomolgus/Rhesus macaque Expression System HEK293 Tag N-His-Avi Expression Range Asn19-Pro152 Mol. Weight The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result. Form Lyophilized Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Endotoxin Less than 1EU per ug by the LAL method. Activity Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 18.4ng/ml