Recombinant Human TIGIT Protein, Active

Product Overview Description Recombinant Human TIGIT Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Met22-Pro141. Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC Accession Q495A1 Target Symbol TIGIT Synonyms TIGIT; VSIG9; VSTM3; WUCAM Species Human Expression System HEK293 Tag C-hFc Expression Range Met22-Pro141 Mol. Weight The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Tris-Bis PAGE result. Form Lyophilized Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Endotoxin Less than 1EU per ug by the LAL method. Activity Immobilized Human TIGIT, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay