2X Taq PCR Master Mix (with Dye)

Background The 2X Taq PCR Master Mix (with dye) is a ready-to-use reagent designed for DNA amplification by polymerase chain reaction (PCR). It contains recombinant Taq DNA polymerase derived from Thermus aquaticus and produced in an E. coli expression system. Taq polymerase catalyzes DNA synthesis by incorporating nucleotides onto primer-template complexes. This polymerase exhibits 5'→3' polymerase activity along with weak 5'→3' exonuclease activity and lacks proofreading (3'→5' exonuclease) functionality, leaving adenine overhangs at amplified fragment 3' ends, facilitating subsequent TA cloning assays. The added dye permits direct loading of PCR products onto agarose gels without additional loading buffers. This reagent is suitable for routine molecular biology applications including genotyping, cloning, and DNA sequence analysis, assisting researchers to reduce experimental variability by minimizing handling steps. Specification Item Value Product Name 2X Taq PCR Master Mix (w