3,3',5,5'-Tetramethylbenzidine
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3,3',5,5'-Tetramethylbenzidine

$27.00
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Description 3,3’,5,5’-Tetramethylbenzidine (TMB) is a sensitive colorimetric substrate widely used in peroxidase and peroxidase-coupled systems such as ELISA. Upon oxidation, TMB produces a blue soluble product, readable spectrophotometrically at 370 nm or 655 nm. The reaction can be stopped with 2 M sulfuric acid, shifting the absorbance to 450 nm and enhancing sensitivity by 2–4 times. Synonyms:BM Blue, SureBlue TMB, TMB Substrate, TMB Blotting Plus Specifications Grade High Purity Formula C16H20N2 C16H20N2 240.4 CAS 54827-17-7 Appearance White or light yellow crystal Assay (HPLC) ≥99.0% Melting point 168.0 – 171.0℃ TLC One spot Heavy metals ≤5ppm Loss on drying ≤0.5% Storage 4℃   Documents COA SDS   Disclaimer: For laboratory research use only.

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