Human Cholesterol-25-Hydroxylase (CH25H) ELISA Kit

Principle of the Assay The microtiter plate provided in this kit has been pre-coated with an antibody specific to CH25H. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CH25H. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain CH25H, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CH25H in the samples is then determined by comparing the O.D. of the samples to the standard curve.For Use with serum, plasma, and cell culture supernatants. For Research Use Only. Not for use in diagnostic procedures. Target Information Catalyzes the formation o