DSAU crosslinker for mass spectrometry

DSAU (disuccinimidyl diacetic urea), also known as ureido-2,2´-diacetic acid bis(hydroxysuccinimide) ester It is a urea-based crosslinker used to study protein structures and interactions by means of Cross-Linking Mass Spectrometry (XL-MS). This symmetrical urea derivate with two N-hydroxysuccinimide (NHS) ester functions reacts rapidly and irreversible with amino functions in proteins, mainly neighboring lysine groups. The chemical chain between both groups defines the distance between the crosslinked lysine functions and can have impact on the yield of intramolecular or intermolecular bioconjugates. The crosslinked protein contains the MS-cleavable urea moiety, which enables unambiguous identification of crosslinked proteins by tandem MS in combination with gas-fragmentation methods. Overview of the available crosslinkers is shown in the table below. DSAU DSPU DSBU PhoX Type NHS ester Urea derivate NHS ester Urea derivate NHS ester Urea derivate NHS ester Phosphon