Amoxicillin::BSA Conjugate

Product DescriptionAmoxicillin is a bactericidal penicillin active against both Gram-positive and Gram-negative organisms and is sensitive to β-Lactamase. It is bactericidal against sensitive organisms during the stage of active multiplication. Amoxicillin exerts its mode of action by interfering with bacterial cell wall synthesis by acylating the enzyme transpeptidase, thus rendering it unable to cross-link muramic acid containing peptidoglycan strands. This inhibition of the biosynthesis of peptidoglycan, a substance necessary for cell wall strength and rigidity, results in a defective cell wall.BSA (bovine serum albumin) is conjugated to amoxicillin (1: 182). The carboxyl group in amoxicillin is directly linked to an amine group in BSA, and/or carboxyl group in BSA is directly linked to the amine group in amoxicillin. Amoxicillin has a molecular weight of 365.4 Da and BSA has a molecular weight of 66.4 kDa. The resultant conjugation solution is then buffer-exchanged with 20 mM PBS,