Sheep Cortisol ELISA

Catalog number CO116S-400 Product type ELISA Quantity 96 Tests (12x8 breakable strip wells) Standard range 1-80 ng/ml Analytical Sensitivity 1 ng/ml Sample volume 40 µl/well Species Sheep Application The Calbiotech Cortisol ELISA Kit is intended for the quantitative measurement of Cortisol in sheep serum or plasma. Principle The Calbiotech Cortisol kit is a solid phase competitive ELISA. The samples and Cortisol enzyme conjugate are added to the wells coated with anti-Cortisol monoclonal antibody. Cortisol in the patient’s sample competes with a Cortisol enzyme conjugate for binding sites. Unbound Cortisol and Cortisol enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Cortisol in the samples. A standard curve is prepared relating color intensity to the concentration of the Cortisol. Storage and Stability Product should be stored at 2-8 °C. Product is stabl