Passage Reagent System™ (4Z0-840)

Passage Reagent System™ a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing plus Attachment Factor™      The Passage Reagent System™ (PRS) is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing, and Attachment Factor™, an extracellular matrix (ECM) product to promote cell attachment to tissue culture surfaces and encourage correct polarity and cytoskeletal organization. The PRS contains four parts: PRG-1™ (EDTA-dPBS Solution), PRG-2™ (Trypsin/EDTA-dPBS Solution),  PRG-3™ (Trypsin Inhibitor-dPBS Solution) and Attachment Factor™ each in a 100mL bottle. The chelating agent EDTA in PRG-1™ prepares for PRG-2™, which contains highly purified trypsin. PRG-3™ inactivates the protease in PRG-2™ and stabilizes the cell membranes. When re-plating, Attachment Factor™ promotes adherence of cells to the tissue culture surface.  Cell membranes are materially and cumulatively damaged w