PRG-2 Trypsin-EDTA Solution (4Z0-310)

PRG-2 (Trypsin-EDTA Solution 1X), 100ml  The Passage Reagent Group™ (PRG) is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1™ (EDTA-dPBS Solution), PRG-2™ (Trypsin/EDTA-dPBS Solution) and PRG-3™ (Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1™ prepares for PRG-2™, which contains highly purified trypsin. PRG-3™ inactivates the protease in PRG-2™ and stabilizes the cell membranes. Cell membranes are materially and cumulatively damaged whenever cells are exposed to serine proteases, physically manipulated, centrifuged, and/or frozen. Use of the Passage Reagent Group (PRG) greatly minimizes damage and stress to cells during passage or freezing of cell cultures. PRG2™ contains highly purified trypsin, allowing a substantial reduction (40%) in the amount of enzyme required to detach cells compared to typical commercial trypsin solutions. Intended for sequential use wit