PRG-3 Trypsin Inhibitor Solution (4Z0-410)

PRG-3 (Trypsin Inhibitor Solution 1X),  100ml    The Passage Reagent Group™ (PRG) is a matched set of Cell Systems Certified™ reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts: PRG-1™ (EDTA-dPBS Solution), PRG-2™ (Trypsin/EDTA-dPBS Solution) and PRG-3™ (Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1™ prepares for PRG-2™, which contains highly purified trypsin. PRG-3™ inactivates the protease in PRG-2™ and stabilizes the cell membranes. Cell membranes are materially and cumulatively damaged whenever cells are exposed to serine proteases, physically manipulated, centrifuged, and/or frozen. Use of the PRG greatly minimizes damage and stress to cells during passage or freezing of cell cultures.  PRG-3™ is intended for sequential use with PRG-1™ and PRG-2™. PRG-3™ inhibits the trypsin activity of PRG-2™, preventing nonspecific protease damage to cell membranes after detachment.  Instructions for use Click to view th