CRISPR Human Genome 80K Knockout Library Module B

Cellecta’s Single-module Human Genome-Wide CRISPR Knockout Library is designed to be small enough (~80,000 constructs) to enable convenient genome-wide “dropout viability” screens with most standard human cell modules. With 4 sgRNAs targeting each gene, all of which have been designed using the criteria for effective guides described by Doench, et al. and containing the Cellecta-designed HEAT sgRNA tracrRNA structure which we have proven generates more robust screening results than the standard sgRNA design, the library has deep enough gene coverage to generate efficient results. Single library with ~80,000 constructs targeting approximately 19,000 protein-coding genes Each gene is targeted by 4 sgRNAs designed based on Doench, et al. criteria All human protein-coding genes coverage—over 19,000 gene targets This library can be obtained with RFP and Puro markers OR GFP and Puro markers. We also have a second Single-module Human Genome-Wide CRISPR Library (Knockout-B Library). Th