Human Genome-Wide CRISPRa Dual-sgRNA Library

Pooled library of ~105,000 dual-sgRNA constructs each targeting a single gene. Designed to work with deactivated spCas9 (dCas9) complexed with an activator (e.g., dCas9-VPH) to activate gene expression. Five CRISPRa dual-sgRNA constructs target each gene. Designs based on Horlbeck, et al., eLife 2016;5:e19760 DOI: 10.7554). Manual and Product Documentation Manual: CRISPR Pooled Lentiviral sgRNA Libraries Gene List: Cellecta Gene List, Human Genome-Wide CRISPRa Dual-sgRNA Library Flyer: CRISPRa and CRISPRi Human Genome-Wide Libraries Library Vector Information * Sequences are available in FASTA (.txt), GenBank (.gb), and SnapGene (.dna) formats. You may use the free SnapGene Viewer to open .dna files. If you would like vector files in another format, please contact us. To download .txt and .gb files, right click on the link and Save As. Search Product Certificates by Lot# or Catalog# Alignment Software Supporting Files Library Configuration File (JSON format, compr