LentiFuge™ Viral Concentration Reagent

Cellecta LentiFuge™ Viral Concentration Reagent provides a simple, rapid, and effective method to increase the amount of lentiviral particles collected from cell culture supernatants using standard high-speed centrifugation. It works by aggregating viral particles so that upon high-speed centrifugation, the virus can be precipitated and isolated without use of an ultracentrifuge. LentiFuge is a sterile, cell culture grade reagent that is non-toxic to target cells. Lentiviral vectors concentrated using LentiFuge contain significantly less culture- medium serum contaminants than those concentrated using polyethylene glycol (PEG)-based reagents, and hence the virus pellet can be resuspended in a much smaller volume. In addition, LentiFuge only requires a 1-hour incubation at 4°C as opposed to lengthy overnight incubations required by PEG reagents. The subsequent centrifugation step in a Beckman JA-14, JA-10, or similar rotor requires an additional hour. After these two short steps, the vi