Tet-Inducible CRISPR Cas9 Lentiviral Vector

All-in-one Tetracycline-Inducible CRISPR Cas9 Lentiviral Vector.  The tet-inducible Cas9 construct contains all the elements needed for transduction and stable integration of an inducible Cas9 gene for CRISPR-mediated gene editing. A. The first option is for a construct expressing Cas9 when doxycycline is present in the cell medium. The Cas9 protein can be expressed for a limited period to catalyze genomic editing and then shut down for subsequent use of the engineered cells. This is available in plasmid or packaged formats. B. The second option is a construct expressing Cas9-BFP fusion when doxycycline is present. This option allows real-time monitoring of induction by measuring BFP fluorescence. This is available in plasmid or packaged formats. The vectors use the same optimized hybrid tet-regulated transactivator rtTA that forms the basis of Cellecta’s InDOXible Tet-Activated Lentiviral Expression System. Cellecta also can make stable custom cell lines with robustly inducible and ti