Alexa Fluor® 488 anti-H2A.X

Histone subunit H2A, along with subunits 2B, 3, and 4, make up the core subunits of the nucleosome octomer. An octomer contains two protomers of each subunit tightly wrapped around a ~147 bp segment of DNA. Histones have integral roles in chromatin integrity, genomic stability, and gene regulation. Post-translational modification of histones in response to certain stimuli results in alterations of nucleosomal positioning relative to DNA. Histone H2A.X is a non-allelic variant of Histone 2A that harbors a C-terminal extension and is essential for checkpoint mediated cell cycle arrest and DNA double-stranded break (DSB) repair in response to both endogenous and exogenous agents, as well as meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation of C-terminal residue serine 139 by ATM (γ-H2A.X) results in the recruitment of DSB-repair machinery. Phopshorylation of H2A.X is also critical for chromatin fragmentation during apoptosis.PRODUCT DETAILSClo