Artocarpus integrifolia Lectin (AIA) - Cy3

Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography (SKU: 21510001) and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the α-chain. The protein's structure explains its carbohydrate-binding specificity of T-antigen disaccharide Galß1,3GalNAc. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal and 2-deoxy-α-D-galactose. Cy3 can be used to viualize the binding pattern of Artocarpus integrifolia Lectin in cellular imaging and flow cytometry. Cy3 is more photostable than many other flourophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling. Specifications: Source: Artocarpus integrifolia (Jackfruit) Activity: Less than 0.5 ug /ml will agglutinate neuraminidase treated human erythrocytes. Protein Concentration (Based on OD 280): 1 mg purified AIA- Cy3 / 1 mL buffer Carbohydrate Specificity: Galß1-3GalNAc Inhibitory Carbohydrate: Galactose or Melibiose Divalent ions required: None Mitogenic Activity: Yes Conjugation: Cy3 Excitation: Maximum at 550 nm Emission: Maximum at 570 nm Fluorescence: Orange Storage and Stability: Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.