AP Biology Investigation #9 - Biotechnology: Restriction Enzyme Analysis of DNA

Students will perform the electrophoresis process on DNA samples treated with different restriction enzymes and construct a standard curve using a known DNA sample While performing the process they will also determine the approximate size of the DNA fragments in the unknown samples Materials provided for eight lab groups Meets AP Science Practices 3 and 6, and Big Idea 3 Innovating Science AP Biology Investigation #9 developed this lab for students to understand the action and specificity of restriction enzymes, while learning the components involved in the process of DNA electrophoresis and the role of each component as it applies to the entire process of electrophoresis. Kit includes: Prepared agarose 0.8%, TBE buffer 5X, DNA stain 10X and DNA samples (DNA marker Lambda DNA HindIII digest, Lambda DNA, Lambda DNA BstEii digest, Lambda DNA EcoRI digest). Safety Data SheetDownload HereItems NeededNot included in the kit:Agarose electrophoresis chamberElectrophoresis power supplyMicrop