I-Blue Midi Plasmid Kit (Endotoxin-Free)

The I-Blue Midi Plasmid Endo-Free Kit (Endotoxin Free) uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50 – 150 ml of cultured bacterial cells. I-Blue Lysis Buffer (an option color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and SDS precipitation. The I-Blue lysis buffer is added during the re-suspension step, then PM2 buffer is added.