3-D Life FG-Dextran Hydrogel Component

Description 3-D Life FG-Dextran is a component of the 3-D Life Hydrogel system. It contains maleimide-functionalized dextran (Mal-Dextran) that can be used together with PEG-Link (Cat. No. L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up fast gelling, cell-compatible hydrogels. When crosslinked with PEG-Link or CD-Link, Dextran-based 3-D Life Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. NOTE: Dextran hydrogels crosslinked with HyLink cannot be dissolved by dextranase. The included 10x CB Buffer (pH 5.5) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells. For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide, which may be downloaded below. Application 3D cell cult