Neuropan-27 Supplement (50x), Defined Serum Substitute for Serum-Free Culture of CNS Neuronal Cells

Introduction Neuropan-27 is provided as a 50x concentrated serum substitute designed for the long-term growth and survival of hippocampal and other neuronal cells of the Central Nervous System (CNS). By supplementing Neuropan Basal Medium with Neuropan-27 and 0.5 mM L-Glutamine, excellent long-term viability of rat embryonic hippocampal neurons has been achieved after several weeks in culture. Glial cell growth is reduced to less than 0.1% for a largely pure neuronal cell culture. When using Neuropan-27 as a supplement to the Neuropan Basal Medium, we suggest to add 25 µM (2.7 µg/ml) L-glutamate to the medium for initial plating of primary hippocampal neurons. Medium changes after day 4 should be done without glutamate. When culturing neuroblastomas, glutamate should be included for both plating and subsequent culturing of cells. Improved long-term survival of hippocampal neurons may be obtained by the addition of beta-mercaptoethanol at 15-30 µM. Application When used as a supplement