PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in PBS, w/o: Ca and Mg

Description PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a 10x concentrated PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. Suitability Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. Specifications Cat. No. P10-024100 Ca/Mg Content: Without Ca and Mg Concentration: 10x concentra