PAN-Biotech L-Glutamine–Penicillin–Streptomycin (200 mM L-Glutamine, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin, in 0.9% NaCl)

Introduction PAN-Biotech L-Glutamine–Penicillin–Streptomycin solution contains 200 mM L-Glutamine, 10,000 U/ml Penicillin, 10 mg/ml Streptomycin, in 0.9% NaCl. The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures to due their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria. L-Glutamine is an essential amino acid required by all mammalian and insect cells grown in culture. It serves as an additional source of carbon and energy and in particular is used for nucleic acid synthesis. Opt