Recombinant Porcine Trypsin, lyophilized powder

Description Our bioactive, Recombinant Porcine Trypsin, lyophilized powder is an enzyme recombinantly expressed in E. coli and purified by standard chromatography techniques. Application Recombinant Porcine Trypsin is used for dissociation of adhesion-dependent cells, and thus may be used to release adherent cells from tissue culture plates for passaging. This animal-free product eliminates the introduction of animal source contaminants found in traditional bovine and porcine trypsin enzymes. For trypsin digestion, the suggested ratio is 1:50 to 1:1000 (w/w). Source Recombinantly produced in E. coli. Formulation Sterile-filtered lyophilized powder. The enzyme was lyophilized with mannitol as preservative. Reconstitution It is recommended to reconstitute the lyophilized Recombinant Porcine Trypsin in sterile 1mM HCl or 50mM HAC not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Biological Activity 4500 USP units/mg protein. One USP unit of trypsi