Biotin rtat HIV-1 IIIB (E.coli)

Product Specifications: Item# 1002-B: Biotin rtat HIV-1 IIIB   Mass/Vial: 50µg   Vol./Vial: 100µl   Diluent: PBS   Stabilizer: 1% BSA  Preservative: Thimersol   Storage: 4°C  Stability: 6 Months    Minimum Dilution: 1 : 1000  Application: T-Cell Research, Diagnostics, Drug Screening, ELISA, Western ELISA.  Description: Biotin conjugated to tat HIV-1 (E.coli). COUPLING RATIO: Approximately 10-20 moles of biotin per mole tat.  Biological Activity: Biotin tat binds to solid phase murine anti-tat mAb as determined by capture ELISA using strepavidin-HRP as a secondary reagent.  Application and Instructions for use:  Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA may be performed in 1 : 1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. tat/anti-tat mAb capture ELISA may