Biotin rnef HIV-1 IIIB (E.coli)

Product Specifications: Item# 1008-B: Biotin rnef HIV-1 IIIB   Mass/Vial: 50µg   Vol./Vial: 50µl  Diluent: PBS   Stabilizer: 1% BSA   Preservative: NaN3,0.1%   Storage: 4°C  Stability: 6 Months  Minimum Dilution: 1 : 2000  Application: T-Cell Research, ELISA, Western ELISA, Drug Screening, Diagnostics.  Description: Biotin conjugated to nef HIV-1 (E.coli).  Coupling Ratio: Approximately 5-10 moles of biotin per mole nef.  Biological Activity: Biotin nef binds to solid phase murine anti-nef mAb as determined by capture ELISA using strepavidin-HRP as a secondary reagent.  Application and Instructions for use:  Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA may be performed in 1 : 1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. nef capture ELISA may be performed in