FLIPR® Calcium 6-QF Assay Kits

This exclusive fluorophore features the highest quantum yield of any calcium indicator on the market, delivering the greatest signal window available in a calcium assay kit. The substantial increase in signal amplitude helps to rekindle low signal screens; including endogenous, primary or stem cell targets. Additionally, the novel dye is unaffected by organic anion exchange proteins, enabling measurement with minimal to no probenecid present. The quench-free (QF) formulation is ideal for quench sensitive targets or assays requiring multiplexing of calcium-flux and luminescent assays within one well. Key Assay Features and Benefits Maximizes fluorescent signal from a wide range of cell types by reducing cell wash artifacts Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput screens Universal mix-and-read protocol accelerates assay workflow and increases throughput Superior signal-to-noise ratio facilitates confirmation of endo