IMAP Assay Components

Based on the specific, high-affinity interaction of phospho groups with trivalent metal-containing nanoparticles (beads), IMAP is a generic, non-antibody-based platform to assess kinase, phosphatase, and phosphodiesterase activity. An enzyme reaction is performed using fluorescently labeled substrate. Addition of the IMAP Binding System stops the enzyme reaction and initiates binding of the beads to phosphorylated substrates. Binding of the substrate to the beads, which correlates to enzyme activity, can be detected using either FP or TR-FRET as a readout. Benefits of IMAP Assays IMAP provides a complete assay system for screening kinases, phosphatases, and phosphodiesterases Because IMAP assays are not antibody-based, they are generic and can be used for any kinase, phosphatase, or phosphodiesterase Robust fluorescence signal gives reliable results with good Z factors IMAP assays are homogeneous and amenable to miniaturization for greater cost savings IMAP assays are available in bot