DMEM-005: DMEM/F-12 Ham 1:1 Mixture 1X, With L-glutamine, Without HEPES

F-12 Nutrient Mixture (Ham’s F-12) is a serum-free medium that relies on a combination of nutrient supplements, growth factors, and hormones to culture cells. During the past decade, researchers have reported successful culture of Leydig cells and Sertoli cells in serum-free medium that contained insulin, transferrin, epidermal growth factor (EGF), leutinizing hormone or follicle stimulating hormone, somatomedin and growth hormone. A 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and Ham’s F-12 Nutrient Mixture was found to be most satisfactory for studies of this type. DME-BBZ-XXX-005 contains 365 mg/L L-glutamine but no HEPES. Storage/Stability The liquid medium should be stored at 2-8°C and stay away from direct sunlight. Deterioration of the liquid medium may be recognized by: (1) Precipitate or particulate matter throughout the solution (2) Cloudy appearance (3) Color change (4) pH change The nature of supplements added may affect storage conditions and shelf life of th