MEM-003: MEM 1X with Earle's Salts, with L-Glutamine, with nonessential amino acids, with HEPES

Minimum Essential Medium (MEM) was developed by Harry Eagle and has been used for a wide variety of cells grown in monolayer. Modified from Basal Medium Eagle (BME) to cultivate fastidious cells like normal mammalian fibroblasts and certain subtypes of HeLa cells, MEM includes higher concentrations of amino acids to achieve a protein composition similar to cultured mammalian cells. MEM containing either Hanks’ or Earle’s salts can be supplemented with non-essential amino acids for use with a variety of cell types. The formulation can be further modified by eliminating calcium, necessary for attachment, to permit growth of cells in suspension culture. MEM-BBZ-XXX-003 is based on Earle’s balanced salts and contains 0.1 mM each (1X) of non-essential amino acids, 100X, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, glycine, L-proline, and L-serine) and 292 mg/L L-glutamine. MEM-BBZ-XXX-003 is used to culture cells that are unable to manufacture or retain non-essential amino aci