Co-IMAC 5 x 3mL Columns

Co-immobilized metal affinity chromatography (IMAC) columns consist of Co-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Co-IMAC Columns separate proteins via their differences in affinity to Co2+ metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag. Technical Specifications Imminodiacetate immobilized matrix charged with Co metal ions. Chelating group coupling method: epoxy activation Matrix: cross-linked Separopore® 4B Particle size (range): 50-170µm Metal ion capacity: > 20µmol Cu2+/ml gel Spacer arm: 14 atom pH stability: 3-12 Binding Capacity: Approx. 40 mg His-tagged protein/ml medium Supplied as suspension in 20% ethanol.