Protein A Separopore® 4B OnePass™ Column

Protein A Separopore® 4B Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Provided as conveniently prepacked, ready-to-use spin columns for gravity flow chromatography. Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics. IP with Protein A • Add 3 ml ice cold RIPA buffer to cell monolayer (or approximately 2–5 x 107 suspension cells in flask) and incubate at 4° C for 10 minutes. For suspension cells, add the RIPA buffer to washed cell pellet in a 15 ml conical centrifuge tube. • Disrupt cells by repeated aspiration through a 21 gauge needle and transfer to a 15 ml conical centrifuge tube. • Wash cell culture plate with additional 1.0 ml ice cold RIPA buffer and combine with original extrac