Reactive Blue-4 Separopore® 4B-CL

Applications: Reactive Blue 4-Separopore is a dye affinity matrix used to purify proteins based on selective binding interactions between certain protein sites and the dye molecule. Differerent immobilized dyes bind from 5 to 60% of the proteins in various crude cell extracts. The affinity for reactive dyes, such as Reactive Blue 4,to proteins may be due to substrate/cofactor similarities as well as hydrophobic and ion exchange properties. Some proteins have been found to require the addition of divalent cations for binding to dye resins. Reactive Blue 4-Separopore® 4B-CL separates proteins based on affinity for this blue dye. Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.