BC-Map555

CLIP-tag is a modified version of the SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Unlike the SNAP-tag, which reacts with benzylguanine (BG)or chloropyrimidine (CP) derivatives, the CLIP-tag is engineered to specifically interact with benzylcytosine (BC)derivatives. This modification allows for distinct labeling applications, enabling researchers to use both SNAP-tag and CLIP-tag in the same experiment, providing flexibility in protein labeling and tracking. BC-Map555 is a CLIP-tag substrate containg Map555 dye and it forms a covalent bond with the CLIP-tag protein, ensuring stable labeling for long-term tracking. MaP555 (558Ex/578Em) is a small-molecule fluorogenic probe with excellent cell permeability. It is converted from 6-carboxytetramethylrhodamine (6-TAMRA) and has similar spectroscopic properties of 6-TAMRA. MaP555 has an acyl sulfamide structure which favors the spirolactam form, thus has excellent cell permeability.