BC-Tetrazine

CLIP-tag is a modified version of the SNAP-tag. It is also a self-labeling protein derived from human O6-alkylguanine-DNA-alkyltransferase. Unlike the SNAP-tag, which reacts with benzylguanine (BG)or chloropyrimidine (CP) derivatives, the CLIP-tag is engineered to specifically interact with benzylcytosine (BC)derivatives. This modification allows for distinct labeling applications, enabling researchers to use both SNAP-tag and CLIP-tag in the same experiment, providing flexibility in protein labeling and tracking. BC-Tetrazine is a CLIP-tag substrate containg a methayltetrazine group for click chemistry applications. Methyltetrazine is typically used in the inverse electron-demand Diels-Alder (iEDDA) reaction, a reaction where tetrazine compounds react with strained dienophiles, such as trans-cyclooctene (TCO) or norbornene derivatives. It is widely used for bioconjugation, fluorescent labeling, and live-cell imaging due to its high reactivity, bioorthogonality, and compatibility w