8 ppt F/2 Medium

8 ppt F/2 Medium Directions For 1 L Total Dilute 1 Liter of seawater to 8 ppt. To approximately 950 mL of the non-pasteurized seawater (8 ppt), add each of the components in the order specified (except vitamins) while stirring continuously. Bring total volume to 1 L with remaining non-pasteurized seawater (8 ppt). For 1.5% agar medium add 15 g of agar into the flask; do not mix. Cover and autoclave medium. When cooled add sterile vitamins. For agar medium add vitamins, mix, and dispense before agar solidifies. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 NaNO3(Fisher BP360-500) 1 mL 7.5 g/100 mL dH20 880 µM 2 NaH2PO4•H2O(MCIB 742) 1 mL 0.5 g/100 mL dH20 36 µM 3 Na2SiO3•9H2O(Sigma 307815) 1 mL 3 g/100 mL dH20 106 µM 4 Trace Metals Solution 1 mL/L 5 Vitamin B12 1 mL/L 6 Biotin Vitamin Solution 1 mL/L 7 Thiamine Vitamin Solution 1 mL/L