Allen Medium

Allen Medium Modification of M.M. Allen's freshwater medium for blue-greens. Suitable for axenic and xenic cultures. The marine LB 1928 Spirulina platensis [Medium: Enriched Seawater (ES)] can be adapted to Allen medium (D. James 1982, pers. Comm.). Directions For 1 L Total To approximately 950 mL of dH2O add each of the components in the order specified while stirring continuously. Adjust pH to 7.8. Bring total volume to 1 L with dH2O. For 1% agar medium: add 10 g of agar to the flask; do not mix. Cover and autoclave medium. Store at refrigerator temperature. # Component Amount Stock Solution Concentration Final Concentration 1 HEPES buffer(Sigma H-3375) 2.3 g/L 0.01 M 2 NaNO3(Fisher BP360-500) 1.5 g/L 0.018 M 3 P-IV Metal Solution 1 mL/L 4 K2HPO4(Sigma P 3786) 5 mL/L 1.5 g/200 mL dH2O 0.215 mM 5 MgSO4•7H2O(Sigma 230391) 5 mL/L 1.5 g/200 mL dH2O 0.152 mM 6 Na2CO3(Baker 3604) 5 mL/L 0.8 g/200 mL dH2O 0.19 mM 7 CaCl2•2H2O(Sigma C-3881) 10 mL/L 0.5 g/200 mL dH