10L Bristol Media Upgrade Kit: Modified Bold 3N Medium

10L Bristol-to-Modified Bold 3N Media Upgrade Kit This upgrade kit includes all the necessary chemical and organic components needed to convert the 10L Bristol Media Kit into 10L of Modified Bold 3N Medium. Modified Bold 3N contains three times (3X) the amount of nitrogen as compared to Bristol, and includes the addition of a soilwater extract component, biotin, thiamine, and vitamin B12. Medium At A Glance Water Type Freshwater pH of Medium 6<pH<8 Contains NO3, Micronutrients, Vitamin B12, Biotin, Thiamine Looking for Modified Bold 3N Medium in 1-Liter and test tube volumes? View/purchase prepared and sterile 1X Modified Bold 3N Medium Directions To upgrade the 10L Bristol Media Kit to Modified Bolds 3N Media: Obtain and wear the appropriate PPE. Prepare the Bristol Media from the 10L Media Kit as directed, except the final volume should be brought up to 9L (9,000 mL) only instead of 10L. Carefully add the solutions (components #1-4) in order from the Media Upgrade Kit to the 9L of Bristol Media. Bring the volume up to 10 Liters with d-H2O and mix well. If needed, autoclave or filter-sterilize the medium and allow to cool to room temperature. Store at refrigerator temperatures. Components In Upgrade Kit Final Concentration NaNO3 8.82 mM P-IV Metal Solution View Recipe Soilwater: GR+ Medium View Recipe Vitamin B12 View Recipe Biotin View Recipe Thiamine View Recipe